Part:BBa_K1523101:Experience

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Applications of BBa_K1523101

Summary:
Our team means to use the part BBa_K216005 to detect the appearance of PNP(p-nitrophenol). So we use K381001 to perform the pre-experiment. We are so happy to find that this part,BBa_K381001, can express green fluorescence when PNP appears.

Methods:
We use the E.Coli DH5 alpha to be the host. The edited bacteria are cultured at 37 centigrade for 18 hours. We next mixed the bacterial liquid 10μl with a series of solutions and we went to see if there would be green fluorescence after 2 hours. We also developed a pathway which can make elctric current by producing riboflavin to sense the PNP. We detect the concentration of riboflavin after culturing in a series of liquid for 2 hours.

Results:

Figure-1 Fluorescence of different system. a.bacterial liquid with 10mM PNP;b.bacterial liquid with ddH2O;c.bacterial liquid with 10mM KNO3;d.bacterial liquid with 10mM KCl.

Figure-2 A sensor pathway we designed.

Figure-3 The riboflavin producing value on the increasing of PNP.

Discussion:

As you can see, the concentration of riboflavin is increasing with the increasing of PNP under 0.5mM. Then it decreased. A reason is that the PNP can kill the strain so they cannot produce riboflavin. While the strain always can live in the 20mM PNP so we should determine the real reason in the future.

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